DMEM(低糖，含雙抗) 細胞培養

DMEM是一種含各種氨基酸和葡萄糖的培養基，高糖型有利于細胞停泊于一個位置生長，適于生長較快、附著較困難腫瘤細胞等。該類培養基廣泛應用于疫苗生產和各種初代病毒宿主細胞的細胞培養及單一細胞培養;如A9、3T6、BALB/3T3、COS-1、COS-3、COS-7、L6、WEHI-3b等細胞系的培養。

DMEM培養基的配制方法：

1 制備新鮮三蒸水或Millipore超純水。

2 稱取所需量的干粉培養基，加入約終體積一半的三蒸水中;若配制一個包裝的培養液，在將整個包裝的干粉倒入三蒸水后，需用水洗包裝袋內面2次，倒入培養液中，以保證所有干粉都溶解成培養液。磁力攪拌或人工攪拌使之*溶解。

3 根據包裝袋上的要求補加所需量的碳酸氫鈉;根據實驗需要，添HEPES(5-20mmol/L)、谷氨酰胺和其他特殊物質。

4 加水定容到終體積。

5 必要時用1 mol/L 鹽酸和1 mol/L 氫氧化鈉調節pH。

6 用無菌0.22um濾膜過濾除菌，分裝于無菌血清瓶中，4℃冰箱保存。

配制好的培養液用前加入100U/mL青霉素和100U/mL鏈霉素，并根據需要加入血清(5%-20%)。

DMEM培養基的特點：

（1）氨基酸含量為依格爾培養基的2倍，且含有非必需氨基酸，如甘氨酸等；

（2）維生素含量為依格爾培養基的4倍；

（3）含有糖酵解途徑中的重要物質——丙酮酸；

（4）含有微量的鐵離子。

DMEM培養基應用廣泛。基于DMEM培養基中氨基酸、微生物含量豐富，并具有糖酵解中的丙酮酸和微量Fe離子，因此在細胞培養、疫苗生產等方面的應用為常見。

相關文獻：

 《Effects of C-myc gene silencing on interleukin-1β-induced rat chondrocyte cell proliferation, apoptosis and cytokine expression》 作者:Jian Zou，Xiao-Lin Li，Zhong-Min Shi，Jian-Feng Xue 期刊:Journal of Bone and Mineral Metabolism 影響因子:2.219 PMID:28616752
《Hematopoietic Substrate-1-Associated Protein X-1 Regulates the Proliferation and Apoptosis of Endothelial Progenitor Cells Through Akt Pathway Modulation》 作者:XIN-BIN GUO,XIN DENG,YING WEI 期刊:Wiley Online Library 影響因子: PMID:29139175
《c-Myc induced upregulation of long non-coding RNA SNHG16 enhances progression and carcinogenesis in oral squamous cell carcinoma》 作者:Shangfeng Li, Shengkai Zhang， Jie Chen 期刊:Cancer gene therapy 影響因子:4.681 PMID:30607006
《Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A》 作者:Lingjuan Du, Guojian Li, Yong Yang, Guokai Yang, Jia Wan, Zhenhuan Ma, Yi Hou 期刊:International Journal of Clinical and Experimental Pathology 影響因子:0.205 PMID:
《Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway》 作者:Panpan Zhang,Lingyue Hua,Huan Hou, Xingyue Du,Zhiqiang He, Menghan Liu, Xiaojuan Hu, Nianlong Yan 期刊:International Journal of Molecular Medicine 影響因子:2.928 PMID:30272329
《Molecular characterization of Cyprinid herpesvirus 3 encoded viral interleukin10》 作者:Ping Ouyang，Ruixue Yang，Lizi Yin，Yi Geng，Weiming Lai，Xiaoli Huang，Defang Chen，Jing Fang，Zhengli Chen，Li Tang，Min He，Chao Huang，Wentao Liu，Kaiyu Wang 期刊:Fish & Shellfish Immunology 影響因子:3.298 PMID:30926478
《Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes》 作者:Lixin Li,Juan Wang,Yun Bai,Jingwei Li,Xiuju Yu,Xiaomao Luo,Zhiwei Zhu,Xiaoyan He,Yanjun Dong,Hongquan Li,Haidong Wang 期刊:Acta Histochemica 影響因子:4.239 PMID:31047685
《Label?free microfluidic chip for the identification of mesothelial cell clusters in pleural effusion》 作者:Lili Zhao,Meng Zhao,Yu Yang,Yajun Gu,Fang Zheng,Xuan Wang,Zhiyuan Zheng,Xuguo Sun 期刊:Oncology Letters 影響因子:1.871 PMID:30944642
《Emodin Attenuates Lipopolysaccharide-Induced Injury via Down-Regulation of miR-223 in H9c2 Cells》 作者:Yuping Yang, Zijun Jiang, Dong Zhuge 期刊:International Heart Journal 影響因子:2.226 PMID:30745529
《Downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR‐101‐3p/WEE1 axis》 作者:Xin Chen, Nuobei Zhang 期刊:Cell Biology International 影響因子:2.127 PMID:30488993
《Knockdown of USP28 enhances the radiosensitivity of esophageal cancer cells via the c‐Myc/hypoxia‐inducible factor‐1 alpha pathway》 作者:Zhang Weili， Liu Zhikun，Wang Jianmin， Tian Qingbao 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:30206969
《HDAC2, but not HDAC1, regulates Kv1.2 expression to mediate neuropathic pain in CCI rats》 作者:Zhihua Li, Yanyan Guo, Xiuhua Ren, Lina Rong, Minjie Huang, Jing Cao, Weidong Zang 期刊:Neuroscience 影響因子:3.244 PMID:31022463
《Differentially expressed genes of HepG2 cells treated with gecko polypeptide mixture》 作者:Yi-Meng Duan, Ying Jin, Meng-Li Guo, Leng-Xin Duan, and Jian-Gang Wang 期刊:Journal of Cancer 影響因子:3.182 PMID:30087713
《青蒿琥酯對高糖條件下血管內皮細胞ICAM-1和MMP-9表達的影響》 作者:葛朋飛,姜 濤,宗 瑤,楊雪嬌,馬玉娜,王云霄,孫 哲,曹子群 期刊:Hans Journal of Surgery 影響因子: PMID:
《Overexpression of microRNA-183 promotes apoptosis of substantia nigra neurons via the inhibition of OSMR in a mouse model of Parkinson's disease》 作者:Jin-Xia Gao， Yu Li， Sai-Nan Wang， Xing-Chi Chen， Lu-Lu Lin， Hui Zhang 期刊:International Journal of Molecular Medicine 影響因子:2.784 PMID:30431059
《A Host Factor GPNMB Restricts Porcine Circovirus Type 2 (PCV2) Replication and Interacts With PCV2 ORF5 Protein》 作者:Kangkang Guo, Lei Xu, Mengmeng Wu, Yufeng Hou, Yanfen Jiang, Jiangman Lv, Panpan Xu, Zhixin Fan, Ruiqi Zhang, Fushan Xing and Yanming Zhang 期刊:Frontiers in Microbiology 影響因子:4.019 PMID:30671053
《Lipid raft localization of epidermal growth factor receptor alters matrix metalloproteinase-1 expression in SiHa cells via the MAPK/ERK signaling pathway》 作者:Zongfeng Zhang, Lina Wang ,Juan Du ,Yuanbo Li, Huilun Yang, Chenxi Li, Hui Li, Haiyang Hu 期刊:Oncology Letters 影響因子:0.237 PMID:28101233
《Trophoblast glycoprotein promotes pancreatic ductal adenocarcinoma cell metastasis through Wnt/planar cell polarity signaling》 作者:Ping He, Shuheng Jiang, Mingze Ma ,Yang Wang, Rongkun Li, Fang Fang, Guangang Tian ,Zhigang Zhang 期刊:Molecular Medicine Reports 影響因子:1.922 PMID:25738465
《High Expression of IL-4R Enhances Proliferation and Invasion of Hepatocellular Carcinoma Cells》 作者:C Guo, Y Ouyang, J Cai, L Xiong 期刊:The International Journal of Biological Markers 影響因子:1.449 PMID:28665449
《Effect of Mst1 overexpression on the growth of human hepatocellular carcinoma HepG2 cells and the sensitivity to cisplatin in vitro》 作者:C Xu, C Liu, W Huang, S Tu, F Wan 期刊:Acta Biochim Biophys Sin 影響因子:2.224 PMID:23419720
《TINAGL1 promotes hepatocellular carcinogenesis through the activation of TGF-β signaling-medicated VEGF expression》 作者:L Sun, Z Dong, H Gu, Z Guo, Z Yu 期刊:Cancer Management and Research 影響因子:3.702 PMID:30697069
《Selective anticancer activity of the novel steroidal dihydropyridine spirooxindoles against human esophageal EC109 cells》 作者:Xiaoli Shi,Tiantian Qin,Weihua Liu,Xueyan Zhang,Leilei Li,Junfeng Huo,Kairui Zhou,Dongxiao Yang,Yanling Zhang,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影響因子:2.068 PMID:
《共軛亞油酸異構體對胰島素抵抗脂肪細胞消耗葡萄糖的影響》 作者:劉曉華， 劉萍， 李海星， 李超波， 曹郁生 期刊:南昌大學學報(理科版), 影響因子:1.187 PMID:
《蜂膠中 ４ 種黃酮對 ＨｅｐＧ２ 細胞胰島素抵抗的改善作用》 作者:梁霞麗　趙亞周　張紅城　彭文君 期刊:中國農業科技導報 影響因子:1.439 PMID:
《Upregulated lncRNA small nucleolar RNA host gene 1 promotes 1‐methyl‐4‐phenylpyridinium ion‐induced cytotoxicity and reactive oxygen species production through miR‐15b‐5p/GSK3β axis in human dopaminergic SH‐SY5Y cells》 作者:Na Xie， Jinxing Qi， Shuang Li， Jianzhong Deng， Yuan Chen， Yajun Lian 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:30302821
《Identification of Anticancer Compounds in Gallnuts Through PCA-constructed Secondary Metabolite Map》 作者:Jiayu Gao, Xiao Yang, Jiangxia Hu and Weiping Yin 期刊:International Journal of Pharmacology 影響因子:0.754 PMID:
《miR?9 depletion suppresses the proliferation of osteosarcoma cells by targeting p16》 作者:Song Gao, Jianchao Wang, Shujian Tian, Jianping Luo 期刊:International Journal of Oncology 影響因子:3.571 PMID:31081054
《Long Non-Coding RNA NIFK-AS1 Augments the Oncogenicity of Hepatocellular Carcinoma by Regulating miR-497/BIRC5 Axis》 作者:Yajing Feng,Lu Sun,Yajuan Feng,Zhixian Guo 期刊:SSRN 影響因子: PMID:
《Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress》 作者:Lixin Huang,Min Huang,Mingyue Shen,Pingwei Wen,Ting Wu,Yuzhen Hong,Qiang Yu,Yi Chen,Jianhua Xie 期刊:International Journal of Biological Macromolecules 影響因子:4.784 PMID:31254574
《miR-185 silencing promotes the progression of atherosclerosis via targeting stromal interaction molecule 1》 作者:Ming Fang, Yanfei Li, Yingbiao Wu, Zhongping Ning, Xuejun Wang & Xinming Li 期刊:Cell Cycle 影響因子:3.259 PMID:30784343
《Insulin-like growth factor 2 mRNA binding protein 2 promotes aerobic glycolysis and cell proliferation in pancreatic ductal adenocarcinoma via stabilizing GLUT1 mRNA》 作者:Shan Huang , Zheng Wu , Yunyun Cheng , Wenzhen Wei  Linlin Hao 期刊:Acta Biochimica et Biophysica Sinica 影響因子:2.502 PMID:31089713
《Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression》 作者:Longfei Xiao ， Liangli Song，  Junjie Hu ， Quanwei Zhang ， Yuting Jiang ， Hongwei Duan ， Yong Zhang， Xingxu Zhao ， Yongli Hua 期刊:Journal of Cellular Physiology 影響因子:4.522 PMID:30671954
《Effect of Artesunate on the Expression of ICAM-1 and MMP-9 in Vascular Endothelial Cells under High Glucose Condition》 作者:Pengfei Ge, Tao Jiang, Yao Zong, Xuejiao Yang, Yuna Ma, Yunxiao Wang, Zhe Sun, Ziqun Cao 期刊:Hans Journal of Ophthalmology 影響因子:1.58 PMID:

DMEM(低糖，含雙抗) 細胞培養